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cell-based protein phosphorylation elisa kits (cellular activation of signaling elisa [casetm kit)]  (SuperArray Bioscience Corporation)
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SuperArray Bioscience Corporation cell-based protein phosphorylation elisa kits (cellular activation of signaling elisa [casetm kit)]
Cells were infected at a multiplicity of infection of 10 or left uninfected ( = time point 0). At the indicated time points after infection, the <t>relative</t> <t>phosphorylation</t> levels of the protein kinase Akt were determined by a cell-based protein phosphorylation <t>ELISA.</t> The data are presented as the amount of activated (phosphorylated) Akt protein (pAkt) relative to total Akt protein (tAkt) (panel A). Total Akt levels are additionally depicted in panel B. Data shown are means ± sem of three to seven independent determinations, each performed in triplicate. The asterisks indicate that the difference between ORF4 − Tet + and ORF4 revertant is statistically significant (p<0,05; Two-way ANOVA).
Cell Based Protein Phosphorylation Elisa Kits (Cellular Activation Of Signaling Elisa [Casetm Kit)], supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cells were infected at a multiplicity of infection of 10 or left uninfected ( = time point 0). At the indicated time points after infection, the relative phosphorylation levels of the protein kinase Akt were determined by a cell-based protein phosphorylation ELISA. The data are presented as the amount of activated (phosphorylated) Akt protein (pAkt) relative to total Akt protein (tAkt) (panel A). Total Akt levels are additionally depicted in panel B. Data shown are means ± sem of three to seven independent determinations, each performed in triplicate. The asterisks indicate that the difference between ORF4 − Tet + and ORF4 revertant is statistically significant (p<0,05; Two-way ANOVA).

Journal: PLoS ONE

Article Title: A Gammaherpesvirus Complement Regulatory Protein Promotes Initiation of Infection by Activation of Protein Kinase Akt/PKB

doi: 10.1371/journal.pone.0011672

Figure Lengend Snippet: Cells were infected at a multiplicity of infection of 10 or left uninfected ( = time point 0). At the indicated time points after infection, the relative phosphorylation levels of the protein kinase Akt were determined by a cell-based protein phosphorylation ELISA. The data are presented as the amount of activated (phosphorylated) Akt protein (pAkt) relative to total Akt protein (tAkt) (panel A). Total Akt levels are additionally depicted in panel B. Data shown are means ± sem of three to seven independent determinations, each performed in triplicate. The asterisks indicate that the difference between ORF4 − Tet + and ORF4 revertant is statistically significant (p<0,05; Two-way ANOVA).

Article Snippet: After the initial screening, selected kinases were analyzed in more detail using cell-based protein phosphorylation ELISA Kits (Cellular Activation of Signaling ELISA [CASE™ Kit]) (SuperArray Bioscience Corporation, Frederick, MD) according to the instructions of the manufacturer.

Techniques: Infection, Enzyme-linked Immunosorbent Assay